2016-01-04 · The partial recovery of keg by abi5 indicated that KEG may have substrates other than ABI5 (Liu and Stone, 2010). In fact, KEG also interacts directly with ABF1 and ABF3, and mediates their ubiquitination and degradation by the proteasome (Chen et al., 2013). Similar to abi5, loss of ABF1 and ABF3 partially rescued the keg phenotype after ABA

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In fact, KEG also interacts directly with ABF1 and ABF3, and mediates their ubiquitination and degradation by the proteasome (Chen et al., 2013). Similar to abi5, loss of ABF1 and ABF3 partially rescued the keg phenotype after ABA 2021-03-06 · Encodes a member of the basic leucine zipper transcription factor family, involved in ABA signalling during seed maturation and germination. The Arabidopsis abscisic acid (ABA)-insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes. Although the mechanisms by which the expression of ABI5 is regulated by ABI3 are unknown, a key observation of our work is that ABI5 can complement abi3‐1, whereas ABI3 cannot complement abi5‐4. This strongly suggests that ABI5 acts downstream of ABI3 to arrest growth of germinated embryos by ABA. Disruption of ABI5 increases Arabidopsis ABA‐insensitivity and decreases the expression of many ABA‐responsive genes (Finkelstein and Lynch, 2000b), whereas ABI5‐overexpressing plants were hypersensitive to ABA during seed germination and early seedling development (Lopez‐Molina et al., 2001). WTy35S::HA-ABI5 transgenic seedlings (T3 generation) were treated in liquid MS medium with [32P]orthophosphoric acid [100 mCi (1 Ci 5 37 GBq), 285.5 Ciymg, NEN] for 90 min. Seedlings were further treated with or without 50 mM ABA for 30 min.

In this review, we present the current understanding of the way ABI5 functions as (1) a regulator of abiotic stress responses and (2) an integrator of ABA crosstalk with other phytohormones. The results that are described primarily refer to In this study, we generated a genome‐wide DNA‐binding map of ANAC060, indicating that it directly binds to a G‐box motif. ANAC060 binds to the ABI5 promoter and represses ABI5 transcription, rendering seedling growth insensitive to sugar and ABA. Abscisic acid‐insensitive 5 (ABI5) is an essential and conserved plant basic leucine zipper transcription factor whose level controls seed germination and postgerminative development. It has been demonstrated that activity of ABI5 is transcriptionally and post‐translationally regulated.

In fact, KEG also interacts directly with ABF1 and ABF3, and mediates their ubiquitination and degradation by the proteasome (Chen et al., 2013). Similar to abi5, loss of ABF1 and ABF3 partially rescued the keg phenotype after ABA 2021-03-06 · Encodes a member of the basic leucine zipper transcription factor family, involved in ABA signalling during seed maturation and germination. The Arabidopsis abscisic acid (ABA)-insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes.

Analysis of nitrogen utilization capability during proliferation and maturation of ABSCISIC ACID INSENSITIVE 5 (ABI5) expression and genetically interacts

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ABI5 functions upstream of miR156 to promote juvenile development by affecting the expression of genes in the miR156‐SPL pathway. Therefore, our study uncovers a new role of ABI5 in vegetative development in plants, and implies a role of ABA signaling in vegetative development in Arabidopsis.

2018-02-20 · ABI5 has been implicated as the final inhibitor of seed germination, possibly acting downstream of the GA repressor RGL2 [ 16, 17 ]. The COP9 signalosome (CSN) is a conserved heteromeric protein complex known to regulate the CULLIN-RING family of ubiquitin E3 ligases (CRLs), including the SCF sub-family of E3s [ 21 ]. Abscisic acid‐insensitive 5 (ABI5) is an essential and conserved plant basic leucine zipper transcription factor whose level controls seed germination and postgerminative development. It has been demonstrated that activity of ABI5 is transcriptionally and post‐translationally regulated. Transgenic plants of ABI5-5, ABI5 S42A, ABI5 S145A, and ABI5 S42AS145AT201A were generated by Agrobacterium tumefaciens GV3101-mediated floral infiltration (Clough and Bent, 1998).

qRT‐PCR analyses showed that the transcription levels of ABI5 and RAV1 were markedly decreased in the RAV1‐U abi5 double mutant (Figure S6). Abi5 LLC is a Florida Domestic Limited-Liability Company filed On February 22, 2021. The company's filing status is listed as Active and its File Number is L21000090327. The Registered Agent on file for this company is AB Asset Management LLC and is located at 301 Arthur Godfrey Road, Miami Beach, FL … ABI5 Our initial mapping of ABI5 localized it to the lower arm of chromosome 2, near the phenotypic marker pyrimidine-requiring (py) (Finkelstein, 1994). To generate fine-mapping populations with closely linked recombinations, we out-crossed abi5-1 and abi5-3 (in the Wassilewskija and Colum-bia [Col] backgrounds, respectively) to lines carrying Sumoylation of ABI5 by the ArabidopsisSUMO E3 ligase SIZ1 negatively regulates abscisic acid signaling Kenji Miuraa,b,1, Jiyoung Leec,2, Jing Bo Jina,3, Chan Yul Yooa, Tomoko Miuraa, and Paul M. Hasegawaa,1 aCenter for Plant Environmental Stress Physiology, Purdue University, West Lafayette, IN 47907; bGraduate School of Life and Environmental Sciences, 2016-01-04 2001-04-10 Participates in ABA-regulated gene expression during seed development and subsequent vegetative stage by acting as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters.
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Therefore, our study uncovers a new role of ABI5 in vegetative development in plants, and implies a role of ABA signaling in vegetative development in Arabidopsis. 2016-11-01 The target genes of ABI5 enable further adaptations to abiotic stresses. In this review, we present the current understanding of the way ABI5 functions as (1) a regulator of abiotic stress responses and (2) an integrator of ABA crosstalk with other phytohormones. The results that are described primarily refer to In this study, we generated a genome‐wide DNA‐binding map of ANAC060, indicating that it directly binds to a G‐box motif. ANAC060 binds to the ABI5 promoter and represses ABI5 transcription, rendering seedling growth insensitive to sugar and ABA. Abscisic acid‐insensitive 5 (ABI5) is an essential and conserved plant basic leucine zipper transcription factor whose level controls seed germination and postgerminative development.

2016-01-04 · The partial recovery of keg by abi5 indicated that KEG may have substrates other than ABI5 (Liu and Stone, 2010). In fact, KEG also interacts directly with ABF1 and ABF3, and mediates their ubiquitination and degradation by the proteasome (Chen et al., 2013).
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In this study, we generated a genome‐wide DNA‐binding map of ANAC060, indicating that it directly binds to a G‐box motif. ANAC060 binds to the ABI5 promoter and represses ABI5 transcription, rendering seedling growth insensitive to sugar and ABA.

2014-06-18 2018-02-20 2021-01-26 2020-03-24 Highlights. The high integration of light and ABA signaling promotes the flavonol biosynthesis. ABA-induced flavonol biosynthesis involves HY5, ABI5, and PFG genes.

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To generate fine-mapping populations with closely linked recombinations, we out-crossed abi5-1 and abi5-3 (in the Wassilewskija and Colum-bia [Col] backgrounds, respectively) to lines carrying ABI5 expression is greatly reduced in abi3‐1 mutants, which has low AtEm1 or AtEm6 expression.